A variety of compounds were investigated for use as sulfur sources for the growth of methanogenic bacteria. Methanococcus (Mc.) deltae, Mc. maripaludis, Methanobacterium (Mb.) species GC-2B, GC-3B , and MMY, Methanobrevibacter (Mbr.) ruminantium , and Methanosarcina (Ms.) barkeri strain 227 grew well with sulfide, So, thiosulfate, or cysteine as sole sulfur source. Mbr. ruminatium was able to grow on SO 4 = or SO 3 = , and Ms. barkeri strain 227 was able to grow on SO 3 = , but not on SO 4 = as a sole sulfur source. Mc. jannaschii grew with sulfide, So, thiosulfate or SO 3 = , but not on cysteine or SO 4 = as sole surface source. Mc. thermolithotrophicus, Mc. jannaschii, Mc. deltae , and Mb. thermoautotrophicum strains Marburg and ΔH were able to grow with methanethiol, ethanethiol, n -propanethiol, n -butanethiol, methyl sulfide, dimethyl sulfoxide, ethyl sulfide, or CS2 as a sulfur source, when very low levels (20–30 μ M ) of sulfide were present; no growth occurred on 5–100 μ M sulfide alone. Methanethiol , ethanethiol, and methyl sulfide-using cultures produced sulfide during growth.
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