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Twenty-two polymorphic microsatellites were isolated from the noble scallop, Chlamys nobilis with a partial genomic library enriched for tandem repeat sequences of (CA)12 and (GA)12. Polymorphism of these loci was assessed in a sample of 32 unrelated individuals. The average allele number was 7.23 per locus with a range of 3–19, and the observed and expected heterozygosity varied from 0.032–0.875 (averaging 0.452) and 0.315–0.939 (averaging 0.646), respectively. After Bonferroni correction ( P > 0.0023), 11 of the 22 markers accorded with Hardy–Weinberg equilibrium, and the rest showed significant departure from HWE. Three pairs of loci showed significant linkage disequilibrium ( P C. farreri and Patinopecten yessoensis was detected. These markers would be useful for studies of population genetics, linkage mapping and other relevant research in C. nobilis .
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