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The rhizosphere soil and roots of maize ( Zea mays L.) plants were collected from plain land of Islamabad, from Durgai and from the Shringal Campus of Malakand University. Azospirillum was isolated, identified and characterized by physiological and biochemical tests using QTS (Quick Testing System) miniatured identification system from the 24 h old bacterial culture grown on Lb (liquid broth) and NFM (nitrogen free medium) suspended in saline solution. The rhizosphere and roots of maize plants collected from plain land of Islamabad at the altitude of 800 m contained Azospirillum lipoferum whereas both the roots and rhizosphere of maize plants collected from Durgai at the altitude of 3500 m and Shringal campus of Malakand University at the altitude of 4000 m contained Azospirillum brasilense.
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