The effects of Nitrogen (N) and Plant Growth Promoting Rhizobacteria (PGPR) on growth and development of sunflower ( Helianthus annuus L. var. Hysun-33) grown in the greenhouse under a natural environment were studied. The N-use efficiency of a sunflower crop grown under three N rates (N1 = 0 kg ha−1, N2 = 120 kg ha−1, and N3 = 240 kg ha−1) and three PGPR levels (R1 = 0 kg ha−1, R2 = 30 kg ha−1, and R3 = 60 kg ha−1) were investigated. The maximum amount of N resulted in higher total dry matter production per plant and the effect was prominent from 34 days after sowing (DAS). Seed yields differed significantly among different sunflower crops especially at limiting N supply, with significant shifts according to the N level. N uptake was an important parameter for yield at all N rates. The 240 kg N ha−1 treatments provided the maximum yield, while the oil contents in these treatments of higher yield showed a lower oil content (%). Harvest index was also significantly correlated to yield across N rates; however, its importance depended much on environmental conditions as well. It can be inferred from the study that sunflower crop is well-supplied with respect to growth, development, yield and yield components, to enhance N efficiency and depends very much on the N supply. All the parameters gave maximum results with the increment of N while PGPR regimes had no prominent impact on the sunflower crop, the target environment, and the target yield level grown under a specified controlled glasshouse environment.