[期刊论文][article]

出版年：2024

Abstract Nitro-fatty acids （NFAs） are endogenous lipid mediators capable of post-translational modifications of selected regulatory proteins. Here, we investigated the anti-cancerous effects of nitro-oleic acid （NO₂OA） and its combination with gamma irradiation on different cancer cell lines. The effects of NO₂OA on cell death, cell cycle distribution, or expression of p21 and cyclin D1 proteins were analyzed in cancer （A-549, HT-29 and FaDu） or normal cell lines （HGF, HFF-1）. Dose enhancement ratio at 50?% survival fraction （DER_IC50） was calculated for samples pre-treated with NO₂OA followed by gamma irradiation. NO₂OA suppressed viability and induced apoptotic cell death. These effects were cell line specific but not in general selective for cancer cells. HT-29 cell line exerted higher sensitivity toward NO₂OA treatment among cancer cell lines tested: induction of cell cycle arrest in the G2/M phase was associated with an increase in p21 and a decrease in cyclin D1 expression. Pre-treatment of HT-29 cells with NO₂OA prior irradiation showed a significantly increased DER_IC50, demonstrating radiosensitizing effects. In conclusion, NO₂OA exhibited potential for combined chemoradiotherapy. Our results encourage the development of new NFAs with improved features for cancer chemoradiation.

apoptosis;cancer;cell cycle;gamma irradiation;nitro-fatty acids;radiosensitization