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[期刊论文][BRIEF COMMUNICATION]
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Radio‐adaptive response, individual radio‐sensitivity and correlation of base excision repair gene polymorphism (hOGG1, APE1, XRCC1, and LIGASE1) in human peripheral blood mononuclear cells exposed to gamma radiation
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作 者：
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Sneh M. Toprani;Birajalaxmi Das;
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出版年：2020
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页 码：551 - 559
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出版社：John Wiley & Sons, Ltd.
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摘 要：
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Radio‐adaptive response (RAR) is a biological mechanism, where cells primed with a low dose exhibit reduced DNA damage with a high challenging dose. Single nucleotide polymorphisms (SNPs) of DNA repair genes including base excision repair (BER) pathway are known to be associated with radio‐sensitivity but involvement in RAR is not yet understood. In the present study, attempt was made to correlate genotype frequencies of four BER SNPs [ hOGG1 (Ser326Cys), XRCC1 (Arg399Gln), APE1 (Asp148Glu) and LIGASE1 (A/C)] with DNA damage, repair and mRNA expression level among 20 healthy donors (12 adaptive and 8 nonadaptive). Our results revealed that LIGASE1 ( p = .002) showed significant correlation with DNA damage and mRNA expression level with increasing dose. hOGG1 (Ser326Cys), XRCC1 (Arg399Gln) and LIGASE1 (A/C) polymorphisms showed significant difference with DNA damage (%T) and mRNA expression profile in primed cells among adaptive donors. In conclusion, BER gene polymorphisms play important role in identifying donors with radio‐sensitivity and RAR in human cells.
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关键字：
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adaptive response;alkaline comet assay;base excision repair pathway;DNA damage;DNA repair;human peripheral blood mononuclear cells (PBMC);ionizing radiation;polymorphism;real time quantitative PCR
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所属期刊
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ISSN: 0893-6692
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来自：John Wiley & Sons, Ltd.
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