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It is of sustainable interest to improve the sensitivity and selectivity of the ionization process, especially for direct analysis of complex samples without matrix separation. Herein, four ambient ionization methods including desorption atmospheric pressure chemical ionization (DAPCI), heat‐assisted desorption atmospheric pressure chemical ionization (heat‐assisted DAPCI), microwave plasma torch (MPT) and internal extractive electrospray ionization (iEESI) were employed for comparative analysis of the navel orange tissue samples by mass spectrometry. The volatile organic compounds (e.g. ethanol, vanillin, leaf alcohol and jasmine lactone) were successfully detected by non‐heat‐assisted DAPCI‐MS, while semi‐volatile organic compounds (e.g. 1‐nonanol and ethyl nonanoate) together with low abundance of non‐volatile organic compounds (e.g. sinensetin and nobiletin) were obtained by heat‐assisted DAPCI‐MS. Typical nonvolatile organic compounds [e.g. 5‐(hydroxymethyl)furfural and glucosan] were sensitively detected with MPT‐MS. Compounds of high polarity (e.g. amino acids, alkaloids and sugars) were easily profiled with iEESI‐MS. Our data showed that more analytes could be detected when more energy was delivered for the desorption ionization purpose; however, heat‐sensitive analytes would not be detected once the energy input exceeded the dissociation barriers of the analytes. For the later cases, soft ionization methods such as iEESI were recommended to sensitively profile the bioanalytes of high polarity. Copyright © 2017 John Wiley And Sons, Ltd.
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