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An early and accurate pregnancy diagnosis is a prime tool for the efficient breeding management. During maternal recognition of pregnancy, there is a release of various proteins in the circulation. These proteins may be helpful in diagnosing early pregnancy in animals. Therefore, a study was conducted on the protein profiling in serum of Sahiwal cattle. Blood samples were collected from 30 Sahiwal cows on day 0, 12, 16, 19 and 22 post-breeding. Serum was separated and subjected to 1D SDS PAGE which revealed specific protein band of molecular weight between 33 and 43 kDa and over expression of 66.0 kDa band in pregnant animals from day 16 to 22. Day 19 serum was then subjected to nano-LC–MS/MS. Total 4406 peptide mass spectra were generated leading to identification of 1,416 peptides finally mapping to give 248 proteins. Out of 248 proteins, 127 were upregulated and 54 were downregulated. 17 proteins were highly upregulated having fold change more than 30. There were total 19 proteins in LC–MS which corresponded to the specific bands in 1D SDS PAGE. Gene ontology using PANTHER 15.0 revealed majority of the proteins are having binding property which is necessary during maternal recognition of pregnancy and implantation. Proteins like lactotransferrin, Golgin A4, MYRIP, PKD1 and PWWP domain containing protein MUM1 have the potential to be the biomarker for pregnancy diagnosis and may be used in developing pregnancy diagnostic kit in future.
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