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The present study was conducted to evaluate the effect of insulin on blood levels of sodium and potassium in crossbred cattle. To this end, forty-eight cows were divided into treatment (I, II and III) and control groups each consisting of 12 animals. Insulin was injected @0.25 IU/Kg body weight subcutaneously on day 0-3, 4-7 and 8-11 of estrous cycle in group I, II and III, respectively and PBS was injected in control animals. Blood samples were collected on day 0, 5, 10, 16 and 21 of estrous cycle. Sodium and potassium concentrations were determined in serum. Potassium level varied significantly (P<0.05) between pregnant and non-pregnant animals of group III on day zero of estrous cycle and may be considered as markers of pregnancy. Insulin administration did not affect the serum potassium and sodium level but higher potassium in pregnant animals indirectly shown its positive effect on conception rate.
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