[期刊论文]


Profundirhabdus halotolerans gen. nov., sp. nov., an haloalkaliphilic actinobacterium isolated from seawater of the Mariana Trench

作   者:
Ronghua Liu;Xinyue Wang;Rong Huang;Xiao-Hua Zhang;Xiaolei Wang;

出版年:2023

页    码:006016 - 006016
出版社:Microbiology Society


摘   要:

A Gram-stain-positive, strictly aerobic, rod-shaped actinobacterium, designated strain ZYF776 T , was isolated from seawater of the Mariana Trench collected at a depth of 4000 m. Results of 16S rRNA gene sequence analysis indicated that strain ZYF776 T was a member of the class Nitriliruptoria and closely related to Nitriliruptor alkaliphilus DSM 45188 T (member of the order Nitriliruptorales , 94.94 % sequence similarity) and Egicoccus halophilus KCTC 33612 T (member of the order Egicoccales , 94.46 %). Strain ZYF776 T was catalase-positive and oxidase-negative. Growth occurred at 16–37 °C (optimum, 28 °C), in the presence of 0–13 % NaCl (w/v; optimum, 4 %) and at pH 7.0–10.0 (optimum, pH 8.0). Cell-wall hydrolysates of strain ZYF776 T contained meso -diaminopimelic (peptidoglycan type A1γ), with ribose, rhamnose and a smaller amount of xylose as the cell-wall sugars. The major menaquinone was MK-10. The predominant fatty acids (>10 %) were C 16:0 , C 17:1 ω 8 c and summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ). The polar lipid profile mainly contained diphosphatidylglycerol, phosphatidylglycerol and phosphoglycolipid. The genomic DNA G+C content of strain ZYF776 T was 68.7 mol%. The genome of strain ZYF776 T was about 5.61 Mbp in size, which was larger than those of the reference strains N. alkaliphilus DSM45188 T (5.56 Mbp) and E. halophilus KCTC 33612 T (3.98 Mbp). The average nucleotide identity and digital DNA–DNA hybridization values between ZYF776 T and the related strains N. alkaliphilus DSM 45188 T and E. halophilus KCTC 33612 T were 76.7 and 20.3 % and 75.8 and 20.0 %, respectively. Based on the polyphasic evidence, a novel genus and species with the name Profundirhabdus halotolerans gen. nov., sp. nov. is proposed. The type strain is ZYF776 T (=JCM 33008 T =MCCC 1K03555 T ).



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所属期刊
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
ISSN: 1466-5026
来自:Microbiology Society