[期刊论文]


Dia- and Rok-dependent enrichment of capping proteins in a cortical region

作   者:
Anja Schmidt;Long Li;Zhiyi Lv;Shuling Yan;Jörg Großhans;

出版年:暂无

页    码:暂无
出版社:The Company of Biologists


摘   要:

Rho signaling with its major targets the formin Dia, Rho kinase (Rok) and non-muscle myosin II control turnover, amount and contractility of actomyosin. Much less investigated has been a potential function for the distribution of F-actin plus and minus ends. In syncytial Drosophila embryos Rho1 signaling is high between actin caps, i. e. the cortical intercap region. Capping protein binds to free plus ends of F-actin to prevent elongation of the filament. Capping protein has served as a marker to visualize the distribution of F-actin plus ends in cells and in vitro. Here, we probed the distribution of plus ends with capping protein in syncytial Drosophila embryos. We found that Capping proteins are specifically enriched in the intercap region similar to Dia and MyoII but distinct from overall F-actin. The intercap enrichment of Capping protein was impaired in dia mutants and embryos, in which Rok and MyoII activation was inhibited. Our observations reveal that Dia and Rok/MyoII control Capping protein enrichment and support a model that Dia and Rok/MyoII control the organization of cortical actin cytoskeleton downstream of Rho1 signaling.



关键字:

Actin;Capping protein;Cell cortex;Dia;Drosophila;Embryo;Epithelial domain;Formin;Myosin;Subapical


所属期刊
Journal of Cell Science
ISSN: 0021-9533
来自:The Company of Biologists