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Methylated anthocyanin glycosides were isolated from red Canna indica flower and identified as malvidin 3-O-(6-O-acetyl-beta-d-glucopyranoside)-5-O-beta-d-glucopyranoside (1), malvidin 3,5-O-beta-d-diglucopyranoside (2), cyanidin-3-O-(6''-O-alpha-rhamnopyranosyl-beta-glucopyranoside (3), cyanidin-3-O-(6''-O-alpha-rhamnopyranosyl)-beta-galactopyranoside (4), cyanidin-3-O-beta-glucopyranoside (5) and cyanidin-O-beta-galactopyranoside (6) by HPLC-PDA. Their structures were subsequently determined on the basis of spectroscopic analyses, that is, (1)H NMR, (13)C NMR, HMQC, HMBC, ESI-MS, and UV-vis. Compounds (1-4) were found to be in major quantity while compounds (5-6) were in minor quantity.
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