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Abstract The mating system and seed variation of Acacia hybrid ( A. mangium × A. auriculiformis ) were studied using allozymes and random amplified polymorphic DNA (RAPD) markers, respectively. Multi-locus outcrossing
rate estimations indicated that the hybrid was predominantly outcrossed (mean±s.e. t
m = 0.86±0.01). Seed variation was investigated using 35 polymorphic RAPD fragments. An analysis of molecular variance (AMOVA)
revealed the highest genetic variation among seeds within a pod (66%?0%), followed by among pods within inflorescence (29%?7%),
and the least variation among inflorescences within tree (<1%). In addition, two to four RAPD profiles could be detected among
seeds within pod. Therefore, the results suggest that a maximum of four seeds per pod could be sampled for the establishment
of a mapping population for further studies.
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